FN ISI Export Format VR 1.0 PT J TI Quantitative super-resolution localization microscopy of DNA in situ using Vybrant® DyeCycle™ Violet fluorescent probe AF Żurek-Biesiada, Dominika Szczurek, Aleksander T. Prakash, Kirti Best, Gerrit Mohana, Giriram K. Lee, Hyun-Keun Roignant, Jean-Yves Dobrucki, Jurek W. Cremer, Christoph Birk, Udo AU Żurek-Biesiada, D Szczurek, AT Prakash, K Best, G Mohana, GK Lee, H Roignant, J Dobrucki, JW Cremer, C Birk, U SO Data in Brief BP 157 EP 171 PY 2016 AB Single Molecule Localization Microscopy (SMLM) is a recently emerged optical imaging method that was shown to achieve a resolution in the order of tens of nanometers in intact cells. Novel high resolution imaging methods might be crucial for understanding of how the chromatin, a complex of DNA and proteins, is arranged in the eukaryotic cell nucleus. Such an approach utilizing switching of a fluorescent, DNA-binding dye Vybrant® DyeCycle™ Violet has been previously demonstrated by us (Żurek-Biesiada et al., 2015) [1]. Here we provide quantitative information on the influence of the chemical environment on the behavior of the dye, discuss the variability in the DNA-associated signal density, and demonstrate direct proof of enhanced structural resolution. Furthermore, we compare different visualization approaches. Finally, we describe various opportunities of multicolor DNA/SMLM imaging in eukaryotic cell nuclei. ER